Are there any rules and regulation from SEBI or exchange to ensure brokerage firm has segregated its own money (assets) from investor money (or assets)?

Thus, investor money in trading account is safe (untouched), and nobody including brokerage firm or its representatives have no access to investor money.

If SEBI is not verifying who is verifying to ensure investor capital remain untouched?

If SEBI is already doing audits for this, then how often they perform these audits?

Yes there are rules, recently SEBI/Exchanges have put up stricter rules for this. Check this article. Exchanges audit atleast once a year. 

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