I have 30 share of idea in my demat acct. Yesterday i brought 20 shares now if i sell 20 shares today which share get debited and do i have to pay demat charges

The system calculates in FIFO (first in first out) format so it check T2 first and then T1 .
your 20 shares will be debited today from your demat (demat charges will be there) and the 20 shares will be credited to demat tomorrow

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