Regarding deduction of expenses to calculate gains

As an Investor, can I deduct brokerage, turnover charges, service tax, stamp duty when calculating short term and long term gains? @TAXIQ.IN

Yes you can , you gain is post these expenses only. Exclusion is only for STT

Do we also include stamp duty and sebi charges?

Stamp Duty & Sebi Charges both are cost for trading in equity , You can add them as cost.