When Annual maintenance Charges will be deducted?

I have been using zerodha since 2018. I have openend both Kite & coin accounts. I have some 10K worth share investment on kite and on Coin I have invested around 50K with Current Value of 80K. Will I be charged AMC ? If so, then will it be charged directly on my linked bank account or Application Balance?

Yes, AMC will be applicable and it will be deducted from your trading account balance.

Trading account balance? as in the Zerodha wallet ?

Right, from the balance in your Zerodha account.